Vetinerary Assistant Practice Exam

Which immunologic test is most commonly performed in practice?

1. Western blot

2. ELISA

3. Immunofluorescence

4. Radioimmunoassay

The correct answer is: ELISA

The enzyme-linked immunosorbent assay (ELISA) is the most commonly performed immunologic test in veterinary practice due to its versatility, sensitivity, and ease of use. ELISA is widely employed to detect specific antibodies or antigens in various samples, making it applicable for diagnosing a range of infectious diseases and conditions in animals. Its ability to provide quantitative results is another significant advantage that enhances its utility in clinical settings. Furthermore, ELISA tests can be performed relatively quickly and with a modest amount of sample, which is beneficial in busy veterinary practices. The simplicity of the assay enables veterinary assistants and technicians to carry out the tests effectively without requiring highly specialized training. In contrast, other immunologic tests such as Western blotting, immunofluorescence, and radioimmunoassay, while valuable in certain contexts, typically involve more complex procedures, longer turnaround times, or safety concerns associated with handling radioisotopes. Therefore, ELISA stands out as the primary immunologic test that practitioners utilize in routine diagnostics.